a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. This product is a novel single-component ready-to-use reagent, presenting a light yellow transparent appearance. breaking through the conventional mode of mixing equal volumes of solution A and solution B before use, making the operation more convenient, effectively avoiding reagent loss and cross-contamination during liquid absorption, and improving the consistency and reproducibility of results. In addition, SuperFemto ECL Master Mix has high sensitivity and enables femtogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide.